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dnase treatment  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher dnase treatment
    Dnase Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnase+treatment/Deoxyribonuclease+I/pmc13129545-160-16-23
    Average 99 stars, based on 1 article reviews
    dnase treatment - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation products
    Article Snippet: After media removal, cells were lysed in Guanidine Isothiocyanate Solution (GITC) (Invitrogen; 15577-018). .. RNA was isolated using 384-well glass fiber filter plates (Pall, 5072), and washed twice with RNA wash buffer (60 mM Potassium Acetate (Thermo Scientific Chemicals, J62817AK), 10 mM Tris-HCL (Invitrogen, 15568025), 60% (v/v) Ethanol (Fisher Scientific, BP2818-4)) prior to a 20 min DNAse treatment (Invitrogen, 18047019). .. After DNase treatment, RNA was washed twice with dilute GITC buffer (75% (v/v) ddH 2 O, 25% (v/v) GITC buffer), twice with RNA wash buffer, and eluted using ddH 2 O. RNA quantity and purity were determined using a NanoDrop Lite (Thermo Scientific, NDLPLUSGL).

    cDNA Synthesis:

    Article Title: H 2 O 2 repurposes plant O 2 sensing to regulate post-hypoxia responses.
    Article Snippet: RNA concentration was quantified using a NanoDrop ND-1000 (Thermo Scientific), and RNA integrity was tested on a 1% agarose gel. .. One microgram of total RNA was subjected to DNase Treatment (Thermo Scientific) and retrotranscribed using a qPCRBIO cDNA Synthesis Kit (PCR Biosystems). .. Real-time qPCR was performed with a QuantStudio 5 Real-Time PCR System (Applied Biosystems) using Power SYBR Master Mix (Thermo Fisher Scientific).

    Article Title: Operating CRISPR/Cas12a in a complex nucleic acid sequence background
    Article Snippet: Total RNA was isolated the following day using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instructions, including the on-column DNase I digestion step. .. To ensure complete removal of residual genomic DNA, the eluted RNA was subjected to an additional DNase treatment using the TURBO DNA-freeTM Kit (Invitrogen) under rigorous conditions according to the manufacturer's instructions with an incubation time of 1 h. Complementary DNA (cDNA) synthesis was performed with 100 ng of RNA per sample using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for qPCR use. qPCR was performed using the Luna® Universal qPCR Kit according to the manufacturer’s protocol. ..

    Synthesized:

    Article Title: Description of a chromosomal fosA6 tandem multicopy fragment and its in vivo evolution in consecutive KPC-producing Klebsiella pneumoniae clinical isolates.
    Article Snippet: RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany). .. After DNase treatment with Turbo DNA-free Kit (Invitrogen, Thermo Fisher Scientific), cDNA was synthesized using the Transcriptor First Strand cDNA Month XXXX Volume 0 Issue 0 10.1128/aac.01877-25 2 h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a ac o n 22 A pr il 20 26 b y 24 00 :1 a0 0: 4b a2 :9 d8 3: e8 3a :c 1f 2: da 0d :a df 7 .. Synthesis Kit (Roche, Switzerland).

    Incubation:

    Article Title: Operating CRISPR/Cas12a in a complex nucleic acid sequence background
    Article Snippet: Total RNA was isolated the following day using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instructions, including the on-column DNase I digestion step. .. To ensure complete removal of residual genomic DNA, the eluted RNA was subjected to an additional DNase treatment using the TURBO DNA-freeTM Kit (Invitrogen) under rigorous conditions according to the manufacturer's instructions with an incubation time of 1 h. Complementary DNA (cDNA) synthesis was performed with 100 ng of RNA per sample using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for qPCR use. qPCR was performed using the Luna® Universal qPCR Kit according to the manufacturer’s protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Operating CRISPR/Cas12a in a complex nucleic acid sequence background
    Article Snippet: Total RNA was isolated the following day using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer’s instructions, including the on-column DNase I digestion step. .. To ensure complete removal of residual genomic DNA, the eluted RNA was subjected to an additional DNase treatment using the TURBO DNA-freeTM Kit (Invitrogen) under rigorous conditions according to the manufacturer's instructions with an incubation time of 1 h. Complementary DNA (cDNA) synthesis was performed with 100 ng of RNA per sample using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for qPCR use. qPCR was performed using the Luna® Universal qPCR Kit according to the manufacturer’s protocol. ..



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